LineaRx, an Applied DNA Sciences, Inc. company, delivers enzymatically-produced LineaDNA™ as an alternative to current plasmid-based DNA manufacturing processes with advantages of speed, purity and scalability to support the next generation of nucleic acid-based therapies. Produced using a proprietary, large-scale polymerase chain reaction (“PCR”) based manufacturing platform, LineaDNA allows for the rapid and efficient cell-free production of high-fidelity DNA sequences.
mRNA/IVT Templates
Efficient mRNA synthesis begins with robust, high-quality DNA IVT templates. Our LineaDNA platform utilizes a proprietary PCR-based process to rapidly scale up DNA production in a 100% cell free workflow. LineaDNA is ready for use in IVT without further modification, contains highly homogeneous poly-T tails and is rigorously quality-controlled. When paired with our Linea™ RNAP polymerase, the process maximizes yield and minimizes dsRNA impurities – substances known to elicit off target innate immune responses – thereby enhancing the overall product quality and safety of the IVT process.
DNA Vaccines
DNA vaccine development relies on the rapid generation of high-purity DNA. Our LineaDNA platform speed surpasses plasmid-based DNA with a manufacturing process rate measured in weeks, not months. LineaDNA is free of host genome contamination and non-target DNA sequences
that are found in plasmid DNA, ensuring that each vaccine candidate meets stringent purity standards. Our rapid enzymatic approach significantly streamlines the development process, offering a scalable solution that can adapt to high demand, particularly in pandemic or outbreak scenarios.
Gene Editing
High-precision DNA is paramount for successful gene editing applications. Our enzymatic production process generates high fidelity blunt-ended DNA templates that are crucial for
facilitating precise homology-directed repair (HDR) and can be produced as single stranded or double stranded DNA. This enhanced capability accelerates research and development cycles in genetic engineering and therapeutic interventions.
Personalized Medicine
In the era of personalized medicine, tailored therapies require the rapid production of custom DNA sequences. LineaDNA is designed to deliver high quality bespoke DNA constructs that match the needs of individual patients. Moreover, our unique, rightsized, scaleable GMP facility for LineaDNA can be tailored to support personalized medicine manufacturing for both DNA or mRNA therapies. This specialized infrastructure ensures that every product is manufactured under the highest quality standards while maintaining the speed and efficiency demanded for individualized therapy development.